@conference { ISI:000245886300024, title = {Novel method for the synthesis of siloxane nanowires and filaments using gold nanoparticle catalysts}, booktitle = {International Conference on Nanoscience and Technology}, series = {International Journal of Nanoscience Series}, volume = {4}, number = {5-6}, year = {2005}, note = {International Conference on Nanoscience and Technology, Calcutta, INDIA, DEC 17-20, 2003}, month = {DEC}, pages = {1007-1010}, publisher = {World Scientific Publ Co Pte Ltd, Po Box 128 Farrer Rd, Singapore 9128, Singapore}, organization = {World Scientific Publ Co Pte Ltd, Po Box 128 Farrer Rd, Singapore 9128, Singapore}, address = { Calcutta, India}, abstract = {

A unique polymerization reaction of alkylsilanes to siloxane nanowires, filaments and tubes in presence of gold nanoparticles and water is presented. The gold nanoparticles, which catalyze this reaction also work as templates restricting the shape of the resulting polymers into wires and tubes. High resolution transmission electron microscopy images clearly reveal the presence of gold nanoparticle at the tip of each wire supporting the template hypothesis. Elemental analyses by different methods confirm the stoichiometry to be C18H37SiO1.5, when the alkylsilane used was C18H37SiH3, thus proving that the alkyl group remains intact during the polymerization reaction.

}, keywords = {gold nanoparticles, Nanowires, siloxane polymers}, doi = {10.1142/S0219581X0500398X}, author = {Bhagavatula L. V. Prasad and Stoeva, S. and Sorensen, C. M. and Klabunde, Kenneth J. and Zaikovskii, V.}, editor = {Sanyal, M. K. and Raychaudhuri, A. K. and Chakravorty, D.} } @article { ISI:000268795100001, title = {Acute and subacute toxicity studies of chitosan reduced gold nanoparticles: a novel carrier for therapeutic agents}, journal = {Journal of Biomedical Nanotechnology}, volume = {5}, number = {3}, year = {2009}, month = {JUN}, pages = {233-239}, publisher = {AMER SCIENTIFIC PUBLISHERS}, address = {25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USA}, abstract = {

The objective of the present study was to evaluate the oral toxicity of chitosan reduced gold nanoparticles so as to demonstrate its applicability for drug delivery application. Acute oral toxicity studies in female rats documented no deaths or treatment related complications. The LD(50) value of gold nanoparticles was found to be greater than 2000 mg/kg. In case of sub-acute oral toxicity studies, gold nanoparticles were administered orally to male and female rats for a period of 28-days. At the end of study blood samples were collected for haematology and biochemical analysis. For histopathological analysis, organs of animals were weighed and processed for examination. All animals survived the duration of the study, with no significant changes in clinical signs, body weight, food consumption, hematological parameters, organ weights and histopathological findings. These studies establish that chitosan reduced gold nanoparticles produced no treatment related toxicity in rats following oral administration, thus can be exploited for potential therapeutic applications.

}, keywords = {Acute Toxicity, Chitosan, gold nanoparticles, Sub-Acute Toxicity, Wistar Rats}, issn = {1550-7033}, doi = {10.1166/jbn.2009.1027}, author = {Pokharkar, Varsha and Dhar, Sheetal and Bhumkar, Devika and Mali, Vishal and Bodhankar, Subhash L. and Bhagavatula L. V. Prasad} } @article { ISI:000272797200013, title = {Optical limiting properties of hydrophobic poly(etherimide) membranes embedded with isolated and aggregated gold nanostructures}, journal = {Colloids and Surfaces A-Physicochemical and Engineering Aspects}, volume = {352}, number = {1-3}, year = {2009}, month = {DEC}, pages = {79-83}, publisher = {ELSEVIER SCIENCE BV}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

A simple method to incorporate spherical or aggregated nanostructures of gold into hydrophobic poly(etherimide) membranes is demonstrated and their comparative nonlinear optical properties are investigated. When excited at 532 nm using laser pulses of 7 ns duration, a reduced transmission behaviour in both the cases, viz., polymers embedded with isolated nanoparticles and their aggregates is observed. This fits to a three-photon type nonlinear absorption process and is attributed to excited state absorption occurring in the nanostructures. Interestingly, the nonlinearity is more prominent in the aggregated nanostructures compared to the spherical nanostructures. These materials are potential candidates for optical limiting applications. (C) 2009 Elsevier B.V. All rights reserved.

}, keywords = {gold nanoparticles, Nanoparticle aggregation, Nonlinear optical properties, Optical limiting, Phase transfer, Polymer nanocomposites}, issn = {0927-7757}, doi = {10.1016/j.colsurfa.2009.10.002}, author = {D{\textquoteright}Britto, Virginia and Sandeep, C. S. Suchand and Philip, R. and Bhagavatula L. V. Prasad} } @article { ISI:000293587300003, title = {Biocompatible gellan gum-reduced gold nanoparticles: cellular uptake and subacute oral toxicity studies}, journal = {Journal of Applied Toxicology}, volume = {31}, number = {5}, year = {2011}, month = {JUL}, pages = {411-420}, publisher = {WILEY-BLACKWELL}, address = {COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA}, abstract = {

Currently gold nanoparticles are being explored for drug delivery and other biomedical applications; therefore it is necessary to study the fate of such nanoparticles inside the body. The objective of the present study was to investigate the cellular uptake and toxicity of the gold nanoparticles synthesized using a microbial polysaccharide, gellan gum, as a capping and reducing agent. The cellular uptake of gold nanoparticles was studied on mouse embryonic fibroblast cells, NIH3T3 and human glioma cell line, LN-229. The cellular uptake study indicated that the gellan gum-reduced gold nanoparticles were located in cancer cells (LN-229) while no uptake was observed in normal mouse embryonic fibroblast cells (NIH3T3). The toxicity of the gold nanoparticles was evaluated by carrying out subacute 28 day oral toxicity studies in rats. Subacute administration of gum-reduced gold nanoparticles to the rats did not show any hematological or biochemical abnormalities. The weight and normal architecture of various organs did not change compared with control. The current findings, while establishing the specific uptake of nanoparticles into cancerous cells, also demonstrates that the gellan gum-reduced gold nanoparticles are devoid of toxicity in animals following oral administration. Copyright (c) 2010 John Wiley \& Sons, Ltd.

}, keywords = {cell lines, cellular uptake, Gellan gum, gold nanoparticles, subacute toxicity}, issn = {0260-437X}, doi = {10.1002/jat.1595}, author = {Dhar, Sheetal and Mali, Vishal and Bodhankar, Subhash L. and Shiras, Anjali and Bhagavatula L. V. Prasad and Pokharkar, Varsha} } @article { ISI:000333730900019, title = {Dilution does the trick: role of mixed solvent evaporation in controlling nanoparticle self-assembly}, journal = {Colloids and Surfaces A-Physicochemical and Engineering Aspects}, volume = {447}, year = {2014}, month = {APR}, pages = {142-147}, publisher = {ELSEVIER SCIENCE BV}, type = {Article}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

An easy and convenient way to prepare superlattices of amine capped gold nanoparticles is presented. It is clearly established that solvent evaporation significantly influences the nature of resulting superlattices and critically governs whether monolayer or multilayer superlattices are formed. More specifically, it is demonstrated that dilution of the nanoparticle dispersion with a similar solvent (but with different vapour pressure) is an expedient handle to control the nature of self-assembly. (C) 2014 Elsevier B.V. All rights reserved.

}, keywords = {Evaporation rate, gold nanoparticles, Nucleation, Self-assembly}, issn = {0927-7757}, doi = {10.1016/j.colsurfa.2014.01.070}, author = {Sahu, Puspanjali and Bhagavatula L. V. Prasad} } @article { ISI:000330489500020, title = {In situ synthesized BSA capped gold nanoparticles: effective carrier of anticancer drug methotrexate to MCF-7 breast cancer cells}, journal = {Materials Science \& Engineering C-Materials For Biological Applications}, volume = {34}, year = {2014}, month = {JAN}, pages = {158-167}, publisher = {ELSEVIER SCIENCE BV}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

The proficiency of MTX loaded BSA capped gold nanoparticles (Au-BSA-MTX) in inhibiting the proliferation of breast cancer cells MCF-7 as compared to the free drug Methotrexate (MTX) is demonstrated based on MTT and Ki-67 proliferation assays. In addition, DNA ladder gel electrophoresis studies, flow cytometry and TUNEL assay confirmed the induction of apoptosis by MTX and Au-BSA-MTX in MCF-7 cells. Notably, Au-BSA-MTX: was found to have higher cytotoxicity on MCF-7 cells compared with an equivalent dose of free MTX. The enhanced activity is attributed to the preferential uptake of Au-BSA-MTX particles by MCF-7 cells due to the presence of BSA that acts as a source of nutrient and energy to the rapidly proliferating MCF-7 cells. Moreover, the targeting ability of the drug MTX to the over expressed folate receptors on MCF-7 cells also contributes to the enhanced uptake and activity. Taken together, these results unveil that Au-BSA-MTX could be more effective than free drug for cancer treatment. (C) 2013 Elsevier B.V. All rights reserved.

}, keywords = {Apoptosis, Bovine serum albumin, Cancer therapy, Cytotoxicity, Dual active agents, gold nanoparticles}, issn = {0928-4931}, doi = {10.1016/j.msec.2013.09.004}, author = {Murawala, Priyanka and Tirmale, Amruta and Shiras, Anjali and Bhagavatula L. V. Prasad} }