01999nas a2200301 4500008004100000022001400041245009700055210006900152260000900221300000900230490000800239520101400247653001501261653004401276653000701320653001701327653003501344653001701379100001701396700001701413700002601430700002201456700002101478700003501499700001701534700002101551856012501572 2018 eng d a0254-058400apH dependent sophorolipid assemblies and their influence on gelation of silk fibroin protein0 apH dependent sophorolipid assemblies and their influence on gela cJAN  a9-160 v2033 a

Sophorolipid (SL), a bio-derived surfactant is an excellent gelling agent for natural fibrous protein, silk fibroin (SF) leading to potential biomedical applications. Interaction of SF with SL has been shown to accelerate the formation of hydrogel with the rate being dependent on the form of SL used. Here, we examine the effect of pH on SL-SF interaction and gel formation by employing rheology, fluorescence spectroscopy, SANS and NMR. The results indicate that the size of SL assemblies decrease as pH increases from acidic to alkaline and significantly impacts the association of SL and SF. The association of SF and SL is mainly via hydrophobic interactions, with the SL molecules forming bead like structures along the SF chain. The increased charge on the acidic form of SL at higher pH results in greater repulsion between acidic SL molecules, which are bound to the hydrophobic sites of SF, leading to rapid chain unfolding and subsequent gelation. (C) 2017 Elsevier B.V. All rights reserved.

10aAssemblies10aNuclear magnetic resonance spectroscopy10apH10aSilk fibroin10aSmall angle neutron scattering10aSophorolipid1 aDubey, Parul1 aKumar, Sugam1 aRavindranathan, Sapna1 aVasudevan, Sahana1 aAswal, Vinod, K.1 aRajamohanan, Pattuparambil, R.1 aNisal, Anuya1 aPrabhune, Asmita uhttp://library.ncl.res.in/content/ph-dependent-sophorolipid-assemblies-and-their-influence-gelation-silk-fibroin-protein02492nas a2200289 4500008004100000245009100041210006900132260000800201300001100209520157300220653003001793653002201823653002101845653002101866653001001887653001901897653001601916653001301932653001701945100002101962700001801983700001902001700002202020700001702042700002602059856011702085 2021 eng d00aSilk fibroin and ceramic scaffolds: comparative in vitro studies for bone regeneration0 aSilk fibroin and ceramic scaffolds comparative in vitro studies  cAPR ae102213 a

Synthetic bone void fillers based on calcium ceramics are used to fill cavities in the bone and promote bone regeneration. More recently, silk fibroin (SF), a protein polymer obtained from Bombyx mori silkworm, has emerged as a promising material in bone void filling. In this work, we have compared the safety and efficacy of two types of silk fibroin-based bone void fillers with currently used and commercially available ceramic bone void fillers (based on calcium sulphate, beta tricalcium phosphate, and beta tricalcium phosphate with hydroxyapatite). Further, we have also evaluated these two types of SF scaffolds, which have strikingly different structural attributes. The biocompatibility of these scaffolds was comparable as assessed by cytotoxicity assay, cellular adhesion assay, and immunogenic assay. Ability of the scaffolds to support differentiation of human mesenchymal stem cells (hMSCs) into an osteoblastic lineage was also evaluated in an in vitro differentiation experiment using reverse transcriptase polymerase chain reaction analysis. These results revealed that cells cultured on SF scaffolds exhibit higher expression of early to late markers such as Runx2, BMPs, collagen, osterix, osteopontin, and osteocalcin as compared with ceramic-based scaffolds. This observation was further validated by studying the expression of alkaline phosphatase and calcium deposition. We also show that scaffolds made from same material of SF, but characterized by very different pore architectures, have diverse outcome in stem cell differentiation.

10abeta tricalcium phosphate10abone regeneration10abone void filler10acalcium sulphate10ahMSCs10ahydroxyapatite10aosteoblasts10ascaffold10aSilk fibroin1 aDeshpande, Rucha1 aShukla, Swati1 aSayyad, Raeesa1 aSalunke, Shalmali1 aNisal, Anuya1 aVenugopalan, Premnath uhttp://library.ncl.res.in/content/silk-fibroin-and-ceramic-scaffolds-comparative-vitro-studies-bone-regeneration02197nas a2200241 4500008004100000245009300041210006900134260000800203300001100211490000700222520140600229653001701635653002101652653002301673653002401696653001701720100002101737700001801758700002001776700001701796700001901813856012301832 2022 eng d00aSilk fibroin and recombinant elastin blend nano-coatings for implantable medical devices0 aSilk fibroin and recombinant elastin blend nanocoatings for impl cDEC a1048750 v333 a

Silicone breast implants are conventionally used to reconstruct the shape and size of the breast in breast cancer patients. However, rate of failure of implants due to formation of capsular contracture is significantly high. Recent studies have shown that coating the surface of the implant with a biocompatible polymer can mitigate this risk. Here, we have modified the surface of implant using a biocompatible natural biopolymer silk fibroin (SF). Further, we have developed formulations of SF with a recombinantly produced elastin-like-peptide (ELP) and compared their performance with a known anti-fouling hydrophilic polymer - polyethylene oxide (PEO). Microscopic and spectroscopic characterization confirm the formation of uniform coatings. These coatings have been also characterized for their ability to resists crack formation. Further, the coatings have been evaluated for their biological performance. Our studies show that addition of 25 wt% of ELP to SF significantly enhances the crack resistance for the coatings. In addition, SF/ELP coatings reduce the adsorption of blood plasma proteins by more than 80 %. Preliminary analysis shows that the SF/ELP blend coatings are non-cytotoxic and support adhesion, growth and proliferation of fibroblast cells. The study therefore demonstrates that SF/ELP coatings have the potential to mitigate the risk of breast implant failure.

10aBlood plasma10aCrack resistance10aProtein adsorption10aRecombinant elastin10aSilk fibroin1 aJoseph, Emmanuel1 aKane, Kartiki1 aParekh, Nimisha1 aNisal, Anuya1 aJanorkar, Amol uhttp://library.ncl.res.in/content/silk-fibroin-and-recombinant-elastin-blend-nano-coatings-implantable-medical-devices03054nas a2200289 4500008004100000022001400041245009800055210006900153260000800222300001400230490000600244520215300250653002102403653002202424653002102446653001402467653001002481653001202491653001702503100002102520700001802541700001502559700002302574700001702597700002602614856012402640 2022 eng d a2373-987800aSilk fibroin microparticle scaffold for use in bone void filling: safety and efficacy studies0 aSilk fibroin microparticle scaffold for use in bone void filling cMAR a1226-12380 v83 a

Silk fibroin (SF) is a natural biocompatible protein polymer extracted from cocoons of silkworm Bombyx mori. SF can be processed into a variety of different forms and shapes that can be used as scaffolds to support bone regeneration. Threedimensional (3D) SF scaffolds have shown promise in bone-void -filling applications. In in vitro studies, it has been demonstrated that a microparticle-based SF (M-RSF) scaffold promotes the differentiation of stem cells into an osteoblastic lineage. The expression of differentiation markers was also significantly higher for M-RSF scaffolds as compared to other SF scaffolds and commercial ceramic scaffolds. In this work, we have evaluated the in vitro and in vivo biocompatibility of M-RSF scaffolds as per the ISO 10993 guidelines in a Good Laboratory Practice (GLP)-certified facility. The cytotoxicity, immunogenicity, genotoxicity, systemic toxicity, and implantation studies confirmed that the M-RSF scaffold is biocompatible. Further, the performance of the MRSF scaffold to support bone formation was evaluated in in vivo bone implantation studies in a rabbit model. Calcium sulfate (CaSO4) scaffolds were chosen as reference material for this study as they are one of the preferred materials for bone-void -filling applications. M-RSF scaffold implantation sites showed a higher number of osteoblast and osteoclast cells as compared to CaSO4 implantation sites indicating active bone remodeling. The number density of osteocytes was double for M-RSF scaffold implantation sites, and these M-RSF scaffold implantation sites were characterized by enhanced collagen deposition, pointing toward a finer quality of the new bone formed. Moreover, the M-RSF scaffold implantation sites had a negligible incidence of secondary fractures as compared to the CaSO4 implantation sites (similar to 50% sites with secondary fracture), implying a reduction in postsurgical complications. Thus, the study demonstrates that the M-RSF scaffold is nontoxic for bone-void -filling applications and facilitates superior healing of fracture defects as compared to commercial calcium-based bone void fillers.

10aBiocompatibility10abiological safety10abone void filler10aISO 1099310aM-RSF10aSerioss10aSilk fibroin1 aDeshpande, Rucha1 aShukla, Swati1 aKale, Amod1 aDeshmukh, Narendra1 aNisal, Anuya1 aVenugopalan, Premnath uhttp://library.ncl.res.in/content/silk-fibroin-microparticle-scaffold-use-bone-void-filling-safety-and-efficacy-studies