02068nas a2200265 4500008004100000022001400041245006900055210006900124260009900193300001200292490000800304520117400312653001201486653001001498653002101508653002601529653001501555100002201570700002501592700002201617700002301639700002001662700002101682856009901703 2009 eng d a0308-814600aCultivar relationships in mango based on fruit volatile profiles0 aCultivar relationships in mango based on fruit volatile profiles aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAY a363-3720 v1143 a

Aroma volatiles of mango (Mangifera indica L.) determine consumer acceptability and influence selection, whilst breeding. To assess their variety, composition and possible impact on cultivar relationships, volatile blends of 22 Indian and five non-Indian cultivars were investigated using solvent extraction and gas chromatography. Totally 84 volatiles belonging to various chemical classes were detected. Based on the Cumulative Occurrence of members of these classes, cultivars were grouped as monoterpene or sesquiterpene dominant. a-Pinene, beta-myrcene and beta-caryophyllene were found in all 27 cultivars. For ordination, common compounds with high (relative) concentration provided quantitative characters, whereas the rare and lesser ones provided qualitative (binary) characters; non-Indian cultivars separated from Indian ones but displayed close relations within their groups. In conclusion, mango flavour is dominated qualitatively as well as quantitatively by terpene hydrocarbons; ancient selection of mango cultivars is hinted to be driven by different aroma characters in different parts of the world. (C) 2008 Elsevier Ltd. All rights reserved.

10aFlavour10aGC-MS10aMangifera indica10aMultivariate analysis10aOrdination1 aPandit, Sagar, S.1 aChidley, Hemangi, G.1 aKulkarni, Ram, S.1 aPujari, Keshav, H.1 aGiri, Ashok, P.1 aGupta, Vidya, S. uhttp://library.ncl.res.in/content/cultivar-relationships-mango-based-fruit-volatile-profiles-002000nas a2200265 4500008004100000022001400041245010900055210006900164260009900233300001200332490000800344520106400352653001301416653001201429653001001441653002001451653001301471653001401484100002501498700002201523700002301545700002001568700002101588856012501609 2013 eng d a0308-814600aSpatial and temporal changes in the volatile profile of alphonso mango upon exogenous ethylene treatment0 aSpatial and temporal changes in the volatile profile of alphonso aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcJAN a585-5940 v1363 a

Alphonso is a highly favoured and exported mango cultivar among the vast mango germplasm of India. Being a climacteric fruit, ethylene plays an important role in ripening of mango. For deeper understanding of effect of pre-climacteric ethylene treatment on volatile profiles of Alphonso mango, 26 volatiles were tracked through six ripening stages of pulp and skin of ethylene-treated and control Alphonso fruits. The study revealed accelerated ripening in terms of early appearance of ripening-specific compounds, lactones and mesifuran, upon ethylene treatment. While the level of lactones remained unaffected, the mesifuran level vastly increased upon ethylene treatment. Skin showed high terpene content while pulp had higher amount of lactones compared to skin. This work points towards involvement of ethylene as a natural hormone in the biosynthesis of lactones and furanones in naturally ripened fruits; whereas, an increase in the terpene level during ripening appears to be independent of ethylene. (C) 2012 Elsevier Ltd. All rights reserved.

10aEthylene10aFlavour10aMango10aPre-climacteric10aRipening10aVolatiles1 aChidley, Hemangi, G.1 aKulkarni, Ram, S.1 aPujari, Keshav, H.1 aGiri, Ashok, P.1 aGupta, Vidya, S. uhttp://library.ncl.res.in/content/spatial-and-temporal-changes-volatile-profile-alphonso-mango-upon-exogenous-ethylene-002411nas a2200277 4500008004100000022001400041245011500055210006900170260007900239300001200318490000700330520145300337653001301790653001501803653001201818653001001830653001401840653001301854100002501867700002201892700002601914700002301940700002001963700002101983856012902004 2016 eng d a1073-608500aMolecular cloning and characterization of O-methyltransferase from mango fruit (mangifera indica cv. alphonso)0 aMolecular cloning and characterization of Omethyltransferase fro a999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USAbHUMANA PRESS INCcMAY a340-3500 v583 a

Flavour of ripe Alphonso mango is invariably dominated by the de novo appearance of lactones and furanones during ripening. Of these, furanones comprising furaneol (4-hydroxy-2,5-dimethyl-3(2H)-furanone) and mesifuran (2,5-dimethyl-4-methoxy-3(2H)-furanone) are of particular importance due to their sweet, fruity caramel-like flavour characters and low odour detection thresholds. We isolated a 1056 bp complete open reading frame of a cDNA encoding S-adenosyl-l-methionine-dependent O-methyltransferase from Alphonso mango. The recombinantly expressed enzyme, MiOMTS showed substrate specificity towards furaneol and protocatechuic aldehyde synthesizing mesifuran and vanillin, respectively, in an in vitro assay reaction. A semi-quantitative PCR analysis showed fruit-specific expression of MiOMTS transcripts. Quantitative real-time PCR displayed ripening-related expression pattern of MiOMTS in both pulp and skin of Alphonso mango. Also, early and significantly enhanced accumulation of its transcripts was detected in pulp and skin of ethylene-treated fruits. Ripening-related and fruit-specific expression profile of MiOMTS and substrate specificity towards furaneol is a suggestive of its involvement in the synthesis of mesifuran in Alphonso mango. Moreover, a significant trigger in the expression of MiOMTS transcripts in ethylene-treated fruits point towards the transcriptional regulation of mesifuran biosynthesis by ethylene.

10aEthylene10aexpression10aFlavour10aMango10aMesifuran10aRipening1 aChidley, Hemangi, G.1 aOak, Pranjali, S.1 aDeshpande, Ashish, B.1 aPujari, Keshav, H.1 aGiri, Ashok, P.1 aGupta, Vidya, S. uhttp://library.ncl.res.in/content/molecular-cloning-and-characterization-o-methyltransferase-mango-fruit-mangifera-indica-cv02983nas a2200301 4500008004100000022001400041245008000055210006900135260000900204300000700213490000800220520207700228653001002305653001802315653001202333653001602345653001702361653002802378653001002406100001602416700002602432700002702458700002202485700002802507700002102535700002002556856010502576 2021 eng d a0032-093500aInfluence of domestication on specialized metabolic pathways in fruit crops0 aInfluence of domestication on specialized metabolic pathways in  cFEB  a610 v2533 a

Main conclusionDuring the process of plant domestication, the selection and traditional breeding for desired characters such as flavor, juiciness and nutritional value of fruits, probably have resulted in gain or loss of specialized metabolites contributing to these traits. Their appearance in fruits is likely due to the acquisition of novel and specialized metabolic pathways and their regulation, driven by systematic molecular evolutionary events facilitated by traditional breeding.AbstractPlants change their armory of specialized metabolism to adapt and survive in diverse ecosystems. This may occur through molecular evolutionary events, such as single nucleotide polymorphism, gene duplication and transposition, leading to convergent or divergent evolution of biosynthetic pathways producing such specialized metabolites. Breeding and selection for improved specific and desired traits (fruit size, color, taste, flavor, etc.) in fruit crops through conventional breeding approaches may further alter content and profile of specialized metabolites. Biosynthetic routes of these metabolites have been studied in various plants. Here, we explore the influence of plant domestication and breeding processes on the selection of biosynthetic pathways of favorable specialized metabolites in fruit crops. An orderly clustered arrangement of genes associated with their production is observed in many fruit crops. We further analyzed selection-based acquisition of specialized metabolic pathways comparing first the metabolic profiles and genes involved in their biosynthesis, followed by the genomic organization of such genes between wild and domesticated horticultural crops. Domestication of crop plants favored the acquisition and retention of metabolic pathways that enhanced the fruit value while eliminated those which produced toxic or unfavorable metabolites. Interestingly, unintentional reorganization of complex metabolic pathways by selection and traditional breeding processes has endowed us with flavorful, juicy and nutritionally rich fruits.

10aAroma10aDomestication10aFlavour10aFruit crops10aGene cluster10aSpecialized metabolites10aTaste1 aDar, Saleem1 aDholakia, Bhushan, B.1 aKulkarni, Abhijeet, P.1 aOak, Pranjali, S.1 aShanmugam, Dhanasekaran1 aGupta, Vidya, S.1 aGiri, Ashok, P. uhttp://library.ncl.res.in/content/influence-domestication-specialized-metabolic-pathways-fruit-crops