01761nas a2200229 4500008004100000022001400041245017600055210006900231260007400300300001200374490000700386520083700393653001201230653001201242653002101254653005101275653003001326653001701356100001701373700002101390856012001411 2007 eng d a0141-549200aPurification by silica gel chromatography using dialysis tubing and characterization of sophorolipids produced from Candida bombicola grown on glucose and arachidonic acid0 aPurification by silica gel chromatography using dialysis tubing  aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcFEB a267-2720 v293 a

The yeast Candida bombicola (ATCC 22214) grown on primary carbon source glucose (100 g l(-1)) and secondary carbon, arachidonic acid (2 g l(-1)) produced mixture of sophorolipids up to 1.44 g l(-1). The crude product was a heterogeneous mixture of sophorolipids, which are glycolipids of sophorose linked to the fatty acid through glycosidic bond between omega and omega-1 carbon of arachidonic acid. The derived sophorolipids were isolated by silica gel chromatography using dialysis tubing. The purified sophorolipids were characterized by ESI-MS and FT-IR. Acid hydrolysis of the resolved sophorolipids were characterized by ESI-MS for the presence of 20-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (20-HETE) and 19-hydroxy-5Z,8Z,11Z,14Z-eico-satetraenoic acid (19-HETE), compounds of pronounced pharmaceutical importance.

10a19-HETE10a20-HETE10aarachidonic acid10aelectrospray soft ionization mass spectroscopy10asilica gel chromatography10aSophorolipid1 aShah, Sachin1 aPrabhune, Asmita uhttp://library.ncl.res.in/content/purification-silica-gel-chromatography-using-dialysis-tubing-and-characterization01412nas a2200253 4500008004100000022001400041245015500055210006900210260010200279300001200381490000800393520040600401653002900807653002600836653002500862653001700887100002000904700001400924700002100938700001900959700002100978700003000999856012901029 2008 eng d a0974-362600aIn situ synthesis of ligand reduced/capped silver nanoparticles and the effect of temperature and ligand structure on the size of silver nanoparticles0 aIn situ synthesis of ligand reducedcapped silver nanoparticles a aC V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIAbINDIAN ACAD SCIENCEScJAN a515-5200 v1203 a

We report in situ synthesis of silver nanoparticles using biosurfactants called sophorolipids as reducing and capping agents. We further study the effect of temperature and the structure of sophorolipid on the size of silver nanoparticles obtained. The silver nanoparticles were characterized by UV-visible, transmission electron microscope (TEM) and light scattering (DLS) analysis techniques.

10adynamic light scattering10aNucleation and growth10asilver nanoparticles10aSophorolipid1 aKasture, M., B.1 aPatel, P.1 aPrabhune, Asmita1 aRamana, C., V.1 aKulkarni, A., A.1 aPrasad, Bhagavatula L. V. uhttp://library.ncl.res.in/content/situ-synthesis-ligand-reducedcapped-silver-nanoparticles-and-effect-temperature-and-ligand01844nas a2200217 4500008004100000022001400041245010700055210006900162260007600231300001200307490000600319520104600325653002001371653001101391653001101402653001701413100002601430700001901456700002101475856013001496 2011 eng d a1860-676800aDifferentiation-inducing ability of sophorolipids of oleic and linoleic acids using a glioma cell line0 aDifferentiationinducing ability of sophorolipids of oleic and li aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcMAY a509-5120 v63 a

Sophorolipids are biosurfactants produced by non-pathogenic yeasts. They show structural similarity with the membrane components of mammalian cells, i.e., glycosphingolipids and gangliosides, which are involved in processes such as signaling, oncogenesis, and differentiation. Sophorolipids have been reported to induce differentiation in several leukemic cell lines, cell death via apoptosis in a human liver cancer cell line, and necrosis in a pancreatic adenocarcinoma cell line. Here we report, for the first time, the effects of precursor fatty acids and sophorolipids of oleic and linoleic acids in pure acidic and crude forms on LN-229, a glioma cell line. In response to different sophorolipid forms, various morphological changes were observed, such as formation of long thread-like extensions arising from the ends of the cells, cell alignment, cell elongation and bundle formation in a dose-dependent manner. In this study we present the morphological evidence of the potential of sophorolipids as differentiation inducers.

10aDifferentiation10aGlioma10aLN-22910aSophorolipid1 aJoshi-Navare, Kasturi1 aShiras, Anjali1 aPrabhune, Asmita uhttp://library.ncl.res.in/content/differentiation-inducing-ability-sophorolipids-oleic-and-linoleic-acids-using-glioma-cell-002864nas a2200229 4500008004100000022001400041245010300055210006900158260006700227300001400294490000800308520201300316653002502329653003502354653002102389653001702410653001402427100002602441700002602467700002102493856012002514 2014 eng d a1438-769700aNew yeast isolate Pichia caribbica synthesizes xylolipid biosurfactant with enhanced functionality0 aNew yeast isolate Pichia caribbica synthesizes xylolipid biosurf a111 RIVER ST, HOBOKEN 07030-5774, NJ USAbWILEY-BLACKWELLcAUG a1070-10790 v1163 a

Sophorolipids (SL) belong to a class of glycolipidic biosurfactants suitable for a wide range of applications. The structural diversity in SL gives rise to variation in physicochemical and biological properties. To achieve the less explored head group diversity in sophorolipid structure, a new xylose fermenting yeast Pichia caribbica has been employed for biosurfactant production. The media and fermentation parameters have been optimized to achieve maximum yield of 7.48 g/L. The physicochemical properties of the xylolipid biosurfactant have been assessed. It reduced the surface tension of distilled water from 70 to 35.9 mN/m with the low critical micellar concentration (CMC) 1.0 mg/L as compared to typical SL (reported CMC range 40-100 mg/L). Structural characterization was done using FTIR and HR-MS to identify the structure. 17-L-[(beta-D-xylopyranosyl)-oxy]-Delta 9-heptadecanoic acid correlated to m/z 415 majorly constituted the product. Control experiment was performed in which glucose was provided as the hydrophilic carbon. This product was also subjected to HR-MS analysis to determine its chemical nature and found to be different from xylolipid. Presence of xylose as head group was anticipated to give altered physicochemical and biological activities and indeed a low CMC value and better inhibitory action was demonstrated against Staphylococcus aureus. Practical applications: Sophorolipids produced by microbial sources have several advantageous properties over commercial petroleum-based surfactants including biodegradability, environmentally friendly nature, and low toxicity. Here, we have attempted to modify the hydrophilic head group by incorporating xylose in place of glucose. This molecule will also behave differently in response to different stimuli. Stimuli-responsive surfactants are a class of compounds with applications in stabilization of emulsions, suspensions or foams, drug encapsulation and delivery, hard-surface cleaning, personal care applications.

10aAntibacterial action10aCritical micelle concentration10aPichia caribbica10aSophorolipid10aXylolipid1 aJoshi-Navare, Kasturi1 aSingh, Pradeep, Kumar1 aPrabhune, Asmita uhttp://library.ncl.res.in/content/new-yeast-isolate-pichia-caribbica-synthesizes-xylolipid-biosurfactant-enhanced-001999nas a2200301 4500008004100000022001400041245009700055210006900152260000900221300000900230490000800239520101400247653001501261653004401276653000701320653001701327653003501344653001701379100001701396700001701413700002601430700002201456700002101478700003501499700001701534700002101551856012501572 2018 eng d a0254-058400apH dependent sophorolipid assemblies and their influence on gelation of silk fibroin protein0 apH dependent sophorolipid assemblies and their influence on gela cJAN  a9-160 v2033 a

Sophorolipid (SL), a bio-derived surfactant is an excellent gelling agent for natural fibrous protein, silk fibroin (SF) leading to potential biomedical applications. Interaction of SF with SL has been shown to accelerate the formation of hydrogel with the rate being dependent on the form of SL used. Here, we examine the effect of pH on SL-SF interaction and gel formation by employing rheology, fluorescence spectroscopy, SANS and NMR. The results indicate that the size of SL assemblies decrease as pH increases from acidic to alkaline and significantly impacts the association of SL and SF. The association of SF and SL is mainly via hydrophobic interactions, with the SL molecules forming bead like structures along the SF chain. The increased charge on the acidic form of SL at higher pH results in greater repulsion between acidic SL molecules, which are bound to the hydrophobic sites of SF, leading to rapid chain unfolding and subsequent gelation. (C) 2017 Elsevier B.V. All rights reserved.

10aAssemblies10aNuclear magnetic resonance spectroscopy10apH10aSilk fibroin10aSmall angle neutron scattering10aSophorolipid1 aDubey, Parul1 aKumar, Sugam1 aRavindranathan, Sapna1 aVasudevan, Sahana1 aAswal, Vinod, K.1 aRajamohanan, Pattuparambil, R.1 aNisal, Anuya1 aPrabhune, Asmita uhttp://library.ncl.res.in/content/ph-dependent-sophorolipid-assemblies-and-their-influence-gelation-silk-fibroin-protein01951nas a2200253 4500008004100000022001400041245010000055210006900155260000800224300001200232490000700244520113400251653002101385653001601406653001201422653001701434653001801451100001701469700002601486700001901512700002001531700002101551856012501572 2019 eng d a0973-129600aFormulation and evaluation of wound healing activity of sophorolipid-sericin gel in wistar rats0 aFormulation and evaluation of wound healing activity of sophorol cAPR a123-1270 v153 a

Sericin is a useful by-product of silk processing and is resistant to oxidation and ultraviolet and can absorb and release moisture easily. Sericin has biological activities such as antibacterial, antioxidant, and tyrosinase inhibition. Sophorolipids are microbial extracellular glycolipids produced by resting cells of Candida bombicola. Sophorolipids show excellent skin compatibility and also have antibacterial property. In this study, we have developed a novel formulation consisting of sericin and sophorolipid with calcium alginate as a binding agent. Since both the ingredients are biocompatible and biodegradable, the formulation was tested for wound healing in Wistar rats. A commercial ointment povidone was used as control. The animal group, treated with sericin and sophorolipid cream, showed fast contraction, rapid closure, and healing when compared with control and commercial ointment. These observations were validated with histopathological studies where more fibroblast proliferation, angiogenesis, and keratinization were observed. This is a green, cost-effective formulation for fast wound healing.

10aBiocompatibility10aformulation10asericin10aSophorolipid10awound Healing1 aMore, Snehal1 aKoratkar, Santosh, S.1 aKadam, Navnath1 aAgawane, Sachin1 aPrabhune, Asmita uhttp://library.ncl.res.in/content/formulation-and-evaluation-wound-healing-activity-sophorolipid-sericin-gel-wistar-rats